ExProt DB
Extremophile Protein Database
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Protein Information

 

Protein Details

ExProtDB ID
A.0000H0.04.H01
Name
Ribonuclease HI
Extreme Stable Behaviour
Halostable

Source Organism

ExProtDB ID
A.0000H0.04
Name
Halobacterium
Domain
Archaea
Extremophile Type
Halophile

Protein Function

Nuclease that specifically degrades the RNA of RNA-DNA hybrids; seems to act exonucleolytically on RNA/DNA hybrids. Endonucleolytically removes RNA primers from the Okazaki fragments of lagging strand synthesis on its own. Complements the temperature-sensitive phenotype of an E.coli double rnhA/rnhB (RNase H) disruption mutant.

Protein Classification

EC ID
E3
EC Number
3.1.26.4
Type
HYDROLASE

Optimum Physiochemical Conditions

Extreme-Stability ID
HA03
Optimum Condition
2.5-5.2M NaCl

Protein Sequence

UniProt KB ID
Molecular Weight (Da)
20981.0
Sequence Length
(amino acid numbers)
199
Sequence
MPVVECDIQTARAALADAGASFSDGNSEHELWHADLGDAHAVAYADKLVVQGGSPTDITAVVQPDRGGRVHAYFDGASRGNPGPAAVGWVLVSGDGGIVAEGGDTIGRATNNQAEYDALIAALEAAADFGFDDIELRGDSQLVEKQLTGAWDTNDPDLRRKRVRARELLTGFDDWSITHVPRATNERADALANEALDDA

Protein Parameters

PID
H.P0034
Amino acid content (%)
 
Ala
16.583
Cys
0.503
Asp
12.06
Glu
5.528
Phe
2.513
Gly
10.553
His
2.513
Ile
3.518
Lys
1.508
Leu
7.538
Met
0.503
Asn
3.518
Pro
3.518
Gln
3.015
Arg
6.533
Ser
4.02
Thr
5.025
Val
7.538
Trp
2.01
Tyr
1.508
Amino acid class contents(%)
 
Tiny (A+C+G+S+T)
36.68
Small (A+C+D+G+N+P+S+T+V)
63.32
Aliphatic (A+I+L+V)
35.18
Aromatic (F+H+W+Y)
8.54
Non Polar (A+C+F+G+I+L+M+P+V+W+Y)
56.28
Polar (D+E+H+K+N+Q+R+S)
43.72
Charged (D+E+H+K+R)
28.14
Basic (H+K+R)
10.55
Acidic (D+E)
17.59
Intraprotein interactions (%)
 
Main Chain Main Chain Hydrogen Bonds
14.94
Main Chain Side Chain Hydrogen Bonds
6.62
Side Chain Side Chain Hydrogen Bonds
3.71
Hydrophonic Interactions
5.82
Ionic Interactions
1.71
Salt Bridges
2.01
Aromatic Aromatic Interactions
0.10
Aromatic Sulphur Interactions
0.05
Cation Pi Interactions
0.35
Disulphide Bridge Interactions
0.00

Protein Structure Details

RCSB PDB ID
Structure Resolution (A)
1.4
PMID
Authors
You, D.J., Angkawidjaja, C., Koga, Y., Kanaya, S.
Journal
To be Published
Title
Crystal structure of RNase H1 from halophilic archaeon Halobacterium salinarum NRC-1
Volume
NA
Year
NA

Information Source

UniProt, RCSB PDB and BRENDA